› 2n1g

Solution NMR

Structure of C-terminal domain of human polymerase Rev1 in complex with PolD3 RIR-motif

Released:
DOI: 10.2210/pdb2n1g/pdb
PDB entry 2n1g coloured by chain, ensemble of 20 models, front view.
PDB entry 2n1g coloured by chain, ensemble of 20 models, side view.
PDB entry 2n1g coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis.
Pustovalova Y, Magalhães MT, D'Souza S, Rizzo AA, Korza G, Walker GC, Korzhnev DM
Biochemistry 55 2043-53 (2016)
PMID: 26982350

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172176 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBZ9 (Residues: 1158-1251; Coverage: 8%)
Gene names: REV1, REV1L
Sequence domains: DNA repair protein REV1 C-terminal domain
Structure domains: DNA repair protein Rev1, C-terminal domain
DNA polymerase delta subunit 3 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15054 (Residues: 231-246; Coverage: 3%)
Gene names: KIAA0039, POLD3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

12 review citations

Translesion DNA polymerases in eukaryotes: what makes them tick?
Vaisman et al. (2017)
11 more

5 mentions without citation

Translesion Synthesis: Insights into the Selection and Switching of DNA Polymerases.
Zhao et al. (2017)
4 more