› 2n3t

Solution NMR

Solution structure of the Rpn1 substrate receptor site toroid 1 (T1)

Released:
DOI: 10.2210/pdb2n3t/pdb
PDB entry 2n3t coloured by chain, ensemble of 10 models, front view.
PDB entry 2n3t coloured by chain, ensemble of 10 models, side view.
PDB entry 2n3t coloured by chain, ensemble of 10 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome.
Shi Y, Chen X, Elsasser S, Stocks BB, Tian G, Lee BH, Shi Y, Zhang N, de Poot SA, Tuebing F, Sun S, Vannoy J, Tarasov SG, Engen JR, Finley D, Walters KJ
Science 351 (2016)
PMID: 26912900

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
26S proteasome regulatory subunit RPN1 Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 13.64 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38764 (Residues: 482-612; Coverage: 13%)
Gene names: HRD2, NAS1, RPD1, RPN1, YHR027C
Sequence domains: Proteasome/cyclosome repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 66%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
50 more

1 mention without citation

Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome.
Shi et al. (2016)