› 2n7b

Solution NMR

Solution structure of the human Siglec-8 lectin domain in complex with 6'sulfo sialyl Lewisx

Released:
DOI: 10.2210/pdb2n7b/pdb
PDB entry 2n7b coloured by chain, ensemble of 20 models, front view.
PDB entry 2n7b coloured by chain, ensemble of 20 models, side view.
PDB entry 2n7b coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for sulfation-dependent self-glycan recognition by the human immune-inhibitory receptor Siglec-8.
Pröpster JM, Yang F, Rabbani S, Ernst B, Allain FH, Schubert M
Proc Natl Acad Sci U S A 113 E4170-9 (2016)
PMID: 27357658

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sialic acid-binding Ig-like lectin 8 Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 16.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NYZ4 (Residues: 17-155; Coverage: 29%)
Gene names: SAF2, SIGLEC8
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, G6S, SIA, FUC
Carbohydrate polymer
1 bound ligand:
Ligand 0D8 1 x 0D8
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Eosinophil and mast cell Siglecs: From biology to drug target.
O'Sullivan et al. (2020)
14 more

10 mentions without citation

Current Status on Therapeutic Molecules Targeting Siglec Receptors.
Lenza et al. (2020)
9 more