› 2n8r

Solution NMR

Productive complex between MMP-12 and synthetic triple-helical collagen, revealed through paramagnetic NMR

Released:
DOI: 10.2210/pdb2n8r/pdb
PDB entry 2n8r coloured by chain, front view.
PDB entry 2n8r coloured by chain, side view.
PDB entry 2n8r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Path to Collagenolysis: COLLAGEN V TRIPLE-HELIX MODEL BOUND PRODUCTIVELY AND IN ENCOUNTERS BY MATRIX METALLOPROTEINASE-12.
Prior SH, Byrne TS, Tokmina-Roszyk D, Fields GB, Van Doren SR
J Biol Chem 291 7888-901 (2016)
PMID: 26887942

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153972 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Macrophage metalloelastase Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39900 (Residues: 100-263; Coverage: 36%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)
Collagen triple helix repeat family protein Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 36 amino acids
Theoretical weight: 3.29 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
1 modified residue:
Ligand HYP 24 x HYP

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 59%
Refinement method: Rigid-body docking, Conformer selection
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 citation in other articles

A perspective on structural and computational work on collagen.
Domene et al. (2016)
3 more

1 mention without citation

Path to Collagenolysis: COLLAGEN V TRIPLE-HELIX MODEL BOUND PRODUCTIVELY AND IN ENCOUNTERS BY MATRIX METALLOPROTEINASE-12.
Prior et al. (2016)