› 2nbw

Solution NMR

Solution structure of the Rpn1 T1 site with the Rad23 UBL domain

Released:
DOI: 10.2210/pdb2nbw/pdb
PDB entry 2nbw coloured by chain, ensemble of 10 models, front view.
PDB entry 2nbw coloured by chain, ensemble of 10 models, side view.
PDB entry 2nbw coloured by chain, ensemble of 10 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.
Chen X, Randles L, Shi K, Tarasov SG, Aihara H, Walters KJ
Structure 24 1257-1270 (2016)
PMID: 27396824

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152409 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
26S proteasome regulatory subunit RPN1 Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 13.64 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38764 (Residues: 482-612; Coverage: 13%)
Gene names: HRD2, NAS1, RPD1, RPN1, YHR027C
Sequence domains: Proteasome/cyclosome repeat
UV excision repair protein RAD23 Chain: B
Molecule details ›
Chain: B
Length: 78 amino acids
Theoretical weight: 8.71 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32628 (Residues: 1-78; Coverage: 20%)
Gene names: RAD23, SYGP-ORF29, YEL037C
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 40%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Meddling with Fate: The Proteasomal Deubiquitinating Enzymes.
de Poot et al. (2017)
13 more

2 mentions without citation

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
1 more