› 2nmb

Solution NMR

DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.

Released:
DOI: 10.2210/pdb2nmb/pdb
PDB entry 2nmb coloured by chain, ensemble of 14 models, front view.
PDB entry 2nmb coloured by chain, ensemble of 14 models, side view.
PDB entry 2nmb coloured by chain, ensemble of 14 models, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity.
Li SC, Zwahlen C, Vincent SJ, McGlade CJ, Kay LE, Pawson T, Forman-Kay JD
Nat Struct Biol 5 1075-83 (1998)
PMID: 9846878

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147761 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein numb Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 17.91 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16554 (Residues: 58-207; Coverage: 27%)
Gene names: CG3779, numb
Sequence domains: Phosphotyrosine interaction domain (PTB/PID)
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
PROTEIN (GPPY PEPTIDE) Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 876 Da
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING WITH AMBIGUOUS RESTRAINTS
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Signal-dependent membrane targeting by pleckstrin homology (PH) domains.
Lemmon et al. (2000)
9 more

3 mentions without citation

Prediction of functional sites by analysis of sequence and structure conservation.
Panchenko et al. (2004)
2 more