› 2nmo

X-ray diffraction
1.35Å resolution

Crystal structure of human galectin-3 carbohydrate-recognition domain at 1.35 angstrom resolution

Released:
DOI: 10.2210/pdb2nmo/pdb
PDB entry 2nmo coloured by chain, front view.
PDB entry 2nmo coloured by chain, side view.
PDB entry 2nmo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Slow diffusion of lactose out of galectin-3 crystals monitored by X-ray crystallography: possible implications for ligand-exchange protocols.
Collins PM, Hidari KI, Blanchard H
Acta Crystallogr D Biol Crystallogr 63 415-9 (2007)
PMID: 17327679

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-3 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17931 (Residues: 113-250; Coverage: 55%)
Gene names: LGALS3, MAC2
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 36.315Å b: 57.602Å c: 62.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Dynamics of galectin-3 in the nucleus and cytoplasm.
Haudek et al. (2010)
6 more

8 mentions without citation

Liquid-liquid phase separation and extracellular multivalent interactions in the tale of galectin-3.
Chiu et al. (2020)
7 more