2nn6

X-ray diffraction
3.35Å resolution

Structure of the human RNA exosome composed of Rrp41, Rrp45, Rrp46, Rrp43, Mtr3, Rrp42, Csl4, Rrp4, and Rrp40

Released:
Model geometry
Fit model/data
Source organism: Homo sapiens
Primary publication:
Reconstitution, activities, and structure of the eukaryotic RNA exosome.
Cell 127 1223-37 (2006)
PMID: 17174896

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170319 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Exosome complex component RRP45 Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 39.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06265 (Residues: 1-302; Coverage: 69%)
Gene names: EXOSC9, PMSCL1
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component RRP41 Chain: B
Molecule details ›
Chain: B
Length: 249 amino acids
Theoretical weight: 26.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NPD3 (Residues: 2-245; Coverage: 100%)
Gene names: EXOSC4, RRP41, SKI6
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component RRP43 Chain: C
Exosome complex component RRP46 Chain: D
Exosome complex component RRP42 Chain: E
Exosome complex component MTR3 Chain: F
Exosome complex component RRP40 Chain: G
Exosome complex component RRP4 Chain: H
Exosome complex component CSL4 Chain: I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P432
Unit cell:
a: 307.8Å b: 307.8Å c: 307.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.291 0.344
Expression system: Escherichia coli