› 2nnh

X-ray diffraction
2.6Å resolution

CYP2C8dH complexed with 2 molecules of 9-cis retinoic acid

Released:
DOI: 10.2210/pdb2nnh/pdb
PDB entry 2nnh coloured by chain, front view.
PDB entry 2nnh coloured by chain, side view.
PDB entry 2nnh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Determinants of cytochrome P450 2C8 substrate binding: structures of complexes with montelukast, troglitazone, felodipine, and 9-cis-retinoic acid.
Schoch GA, Yano JK, Sansen S, Dansette PM, Stout CD, Johnson EF
J Biol Chem 283 17227-37 (2008)
PMID: 18413310

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-145365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2C8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 476 amino acids
Theoretical weight: 54.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10632 (Residues: 28-490; Coverage: 95%)
Gene name: CYP2C8
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
3 bound ligands:
Ligand SO4 2 x SO4
Ligand 9CR 4 x 9CR
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21212
Unit cell:
a: 139.81Å b: 163.62Å c: 73.555Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.29
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Role of Cytochrome P450 2C8 in Drug Metabolism and Interactions.
Backman et al. (2016)
17 more

13 mentions without citation

Recent Structural Insights into Cytochrome P450 Function.
Guengerich et al. (2016)
12 more