› 2nr6

X-ray diffraction
2.81Å resolution

Crystal structure of the complex of antibody and the allergen Bla g 2

Released:
DOI: 10.2210/pdb2nr6/pdb
PDB entry 2nr6 coloured by chain, front view.
PDB entry 2nr6 coloured by chain, side view.
PDB entry 2nr6 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a dimerized cockroach allergen Bla g 2 complexed with a monoclonal antibody.
Li M, Gustchina A, Alexandratos J, Wlodawer A, Wünschmann S, Kepley CL, Chapman MD, Pomés A
J Biol Chem 283 22806-14 (2008)
PMID: 18519566

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211313 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Aspartic protease Bla g 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 36.18 KDa
Source organism: Blattella germanica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P54958 (Residues: 25-350; Coverage: 98%)
Sequence domains: Eukaryotic aspartyl protease
Structure domains:
Antibody light chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 211 amino acids
Theoretical weight: 23.34 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
Structure domains: Immunoglobulins
Antibody heavy chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 213 amino acids
Theoretical weight: 22.89 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand ZN 2 x ZN
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 77.004Å b: 103.187Å c: 146.329Å
α: 90° β: 94.83° γ: 90°
R-values:
R R work R free
0.235 0.232 0.281
Expression system: Komagataella pastoris

Quick links

Citations

15 review citations

IgE Antibodies: From Structure to Function and Clinical Translation.
Sutton et al. (2019)
14 more

18 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
17 more