› 2nte

X-ray diffraction
1.9Å resolution

Crystal Structure of the BARD1 BRCT Domains

Released:
DOI: 10.2210/pdb2nte/pdb
PDB entry 2nte coloured by chain, front view.
PDB entry 2nte coloured by chain, side view.
PDB entry 2nte coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the BARD1 BRCT domains.
Birrane G, Varma AK, Soni A, Ladias JA
Biochemistry 46 7706-12 (2007)
PMID: 17550235

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BRCA1-associated RING domain protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 24.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99728 (Residues: 568-777; Coverage: 27%)
Gene name: BARD1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand CL 3 x CL
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P212121
Unit cell:
a: 57.441Å b: 75.797Å c: 116.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.247
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

The Effects of Genetic and Epigenetic Alterations of BARD1 on the Development of Non-Breast and Non-Gynecological Cancers.
Watters et al. (2020)
2 more

11 mentions without citation

BRCT domains: easy as one, two, three.
Leung et al. (2011)
10 more