› 2nwg

X-ray diffraction
2.07Å resolution

Structure of CXCL12:heparin disaccharide complex

Released:
DOI: 10.2210/pdb2nwg/pdb
PDB entry 2nwg coloured by chain, front view.
PDB entry 2nwg coloured by chain, side view.
PDB entry 2nwg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional basis of CXCL12 (stromal cell-derived factor-1 alpha) binding to heparin.
Murphy JW, Cho Y, Sachpatzidis A, Fan C, Hodsdon ME, Lolis E
J Biol Chem 282 10018-10027 (2007)
PMID: 17264079

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Stromal cell-derived factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 68 amino acids
Theoretical weight: 7.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48061 (Residues: 22-88; Coverage: 93%)
Gene names: CXCL12, SDF1, SDF1A, SDF1B
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: SGN, UAP
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 36.492Å b: 56.965Å c: 71.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.24 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Enhancing the migration ability of mesenchymal stromal cells by targeting the SDF-1/CXCR4 axis.
Marquez-Curtis et al. (2013)
16 more

15 mentions without citation

Demystifying heparan sulfate-protein interactions.
Xu et al. (2014)
14 more