› 2nzl

X-ray diffraction
1.35Å resolution

Crystal structure of human hydroxyacid oxidase 1

Released:
DOI: 10.2210/pdb2nzl/pdb
PDB entry 2nzl coloured by chain, front view.
PDB entry 2nzl coloured by chain, side view.
PDB entry 2nzl coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ugochukwu E, Kavanagh K, Pilka E, Berridge G, Debreczeni JE, Papagrigoriou E, Turnbull A, Niesen F, Gileadi O, von Delft F, Sundstrom M, Arrowsmith CH, Weigelt J, Edwards A, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
Glyoxylate + H(2)O + O(2) = oxalate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-193815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-Hydroxyacid oxidase 1 Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UJM8 (Residues: 1-370; Coverage: 100%)
Gene names: GOX1, HAO1
Sequence domains: FMN-dependent dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:
Ligand GLV 1 x GLV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I4
Unit cell:
a: 97.294Å b: 97.294Å c: 79.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.126 0.155
Expression system: Escherichia coli BL21(DE3)

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