› 2o23

X-ray diffraction
1.2Å resolution

The structure of wild-type human HADH2 (17beta-hydroxysteroid dehydrogenase type 10) bound to NAD+ at 1.2 A

Released:
DOI: 10.2210/pdb2o23/pdb
PDB entry 2o23 coloured by chain, front view.
PDB entry 2o23 coloured by chain, side view.
PDB entry 2o23 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kavanagh KL, Shafqat N, Scholer K, Debreczeni J, Zschocke J, von Delft F, Weigelt J, Arrowsmith C, Sundstrom M, Edwards A, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methylacetoacetyl-CoA + NADH
Testosterone + NAD(+) = androstenedione + NADH
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyacyl-CoA dehydrogenase type-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 27.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99714 (Residues: 1-261; Coverage: 100%)
Gene names: ERAB, HADH2, HSD17B10, MRPP2, SCHAD, SDR5C1, XH98G2
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 117.037Å b: 117.037Å c: 69.087Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.123 0.123 0.154
Expression system: Escherichia coli BL21(DE3)

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