› 2o2v

X-ray diffraction
1.83Å resolution

Crystal Structure of the Complex of Human Mitogen Activated Protein Kinase Kinase 5 Phox Domain (MAP2K5-phox) with Human Mitogen Activated Protein Kinase Kinase Kinase 3 (MAP3K3B-phox)

Released:
DOI: 10.2210/pdb2o2v/pdb
PDB entry 2o2v coloured by chain, front view.
PDB entry 2o2v coloured by chain, side view.
PDB entry 2o2v coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Filippakopoulos P, Savitsky P, Ugochukwu E, Edwards A, Arrowsmith C, Sundstrom M, von Delft F, Knapp S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171581 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dual specificity mitogen-activated protein kinase kinase 5 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13163 (Residues: 5-108; Coverage: 23%)
Gene names: MAP2K5, MEK5, MKK5, PRKMK5
Sequence domains: PB1 domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Mitogen-activated protein kinase kinase kinase 3 Chain: B
Molecule details ›
Chain: B
Length: 90 amino acids
Theoretical weight: 10.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99759 (Residues: 36-124; Coverage: 14%)
Gene names: MAP3K3, MAPKKK3, MEKK3
Sequence domains: PB1 domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 87.654Å b: 87.654Å c: 55.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.224
Expression system: Escherichia coli

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