› 2o60

X-ray diffraction
1.55Å resolution

Calmodulin bound to peptide from neuronal nitric oxide synthase

Released:
DOI: 10.2210/pdb2o60/pdb
PDB entry 2o60 coloured by chain, front view.
PDB entry 2o60 coloured by chain, side view.
PDB entry 2o60 coloured by chain, top view.
Source organisms:
Entry authors: Ng HL, Alber T, Wand AJ

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158573 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62149 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CAM, RCJMB04_24e7
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Nitric oxide synthase 1 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.58 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9Z0J4 (Residues: 725-747; Coverage: 2%)
Gene name: Nos1

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 67.126Å b: 32.93Å c: 73.83Å
α: 90° β: 93.3° γ: 90°
R-values:
R R work R free
0.204 0.202 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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