› 2o88

X-ray diffraction
1.75Å resolution

Crystal structure of the N114A mutant of ABL-SH3 domain complexed with a designed high-affinity peptide ligand: implications for SH3-ligand interactions

Released:
DOI: 10.2210/pdb2o88/pdb
PDB entry 2o88 coloured by chain, front view.
PDB entry 2o88 coloured by chain, side view.
PDB entry 2o88 coloured by chain, top view.
Source organisms:
Primary publication:
Crystallization by capillary counter-diffusion and structure determination of the N114A mutant of the SH3 domain of Abl tyrosine kinase complexed with a high-affinity peptide ligand.
Cámara-Artigas A, Palencia A, Martínez JC, Luque I, Gavira JA, García-Ruiz JM
Acta Crystallogr D Biol Crystallogr 63 646-52 (2007)
PMID: 17452790

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132609 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ABL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00519 (Residues: 64-121; Coverage: 5%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH3 domain
Structure domains: SH3 Domains
P41 peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 48.17Å b: 50.093Å c: 56.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.213
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 citation in other articles

pH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum.
Camara-Artigas et al. (2012)
3 more

2 mentions without citation

Role of interfacial water molecules in proline-rich ligand recognition by the Src homology 3 domain of Abl.
Palencia et al. (2010)
1 more