› 2obv

X-ray diffraction
2.05Å resolution

Crystal structure of the human S-adenosylmethionine synthetase 1 in complex with the product

Released:
DOI: 10.2210/pdb2obv/pdb
PDB entry 2obv coloured by chain, front view.
PDB entry 2obv coloured by chain, side view.
PDB entry 2obv coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pilka ES, Shafqat N, Kavanagh KL, Cooper C, Hozjan V, Turnbull A, von Delft F, Arrowsmith CH, Edwards A, Weigelt J, Sundstrom M, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-169403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-1 Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 42.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00266 (Residues: 16-395; Coverage: 96%)
Gene names: AMS1, MAT1A, MATA1
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand SAM 1 x SAM
Ligand PG4 3 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 66.404Å b: 95.016Å c: 115.773Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.139 0.183
Expression system: Escherichia coli

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