› 2oc9

X-ray diffraction
2.59Å resolution

Crystal structure of human purine nucleoside phosphorylase mutant H257G with Imm-H

Released:
DOI: 10.2210/pdb2oc9/pdb
PDB entry 2oc9 coloured by chain, front view.
PDB entry 2oc9 coloured by chain, side view.
PDB entry 2oc9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Neighboring group participation in the transition state of human purine nucleoside phosphorylase.
Murkin AS, Birck MR, Rinaldo-Matthis A, Shi W, Taylor EA, Almo SC, Schramm VL
Biochemistry 46 5038-49 (2007)
PMID: 17407325

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 1-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand IMH 1 x IMH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R32
Unit cell:
a: 142.17Å b: 142.17Å c: 168.674Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.194 0.243
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Binding isotope effects: boon and bane.
Schramm VL. (2007)
2 more

1 mention without citation

Neighboring group participation in the transition state of human purine nucleoside phosphorylase.
Murkin et al. (2007)