› 2omg

X-ray diffraction
1.52Å resolution

Structure of human insulin cocrystallized with protamine and urea

Released:
DOI: 10.2210/pdb2omg/pdb
PDB entry 2omg coloured by chain, front view.
PDB entry 2omg coloured by chain, side view.
PDB entry 2omg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of insulin NPH formulations.
Norrman M, Hubálek F, Schluckebier G
Eur J Pharm Sci 30 414-23 (2007)
PMID: 17339105

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

6 bound ligands:
Ligand NA 1 x NA
Ligand CRS 3 x CRS
Ligand URE 6 x URE
Ligand ARF 5 x ARF
Ligand ZN 1 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-5
Spacegroup: P43212
Unit cell:
a: 61.66Å b: 61.66Å c: 85.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.209

Quick links

Citations

4 review citations

Absorption kinetics of insulin after subcutaneous administration.
Søeborg et al. (2009)
3 more

2 mentions without citation

SNAP predicts effect of mutations on protein function.
Bromberg et al. (2008)
1 more