› 2oo4

X-ray diffraction
2Å resolution

Structure of LNR-HD (Negative Regulatory Region) from human Notch 2

Released:
DOI: 10.2210/pdb2oo4/pdb
PDB entry 2oo4 coloured by chain, front view.
PDB entry 2oo4 coloured by chain, side view.
PDB entry 2oo4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for autoinhibition of Notch.
Gordon WR, Vardar-Ulu D, Histen G, Sanchez-Irizarry C, Aster JC, Blacklow SC
Nat Struct Mol Biol 14 295-300 (2007)
PMID: 17401372

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Notch 2 extracellular truncation Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 25.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04721 (Residues: 1423-1677; Coverage: 10%)
Gene name: NOTCH2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand ZN 1 x ZN
Ligand GOL 10 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, null
Spacegroup: P212121
Unit cell:
a: 45.372Å b: 74.706Å c: 139.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.268
Expression system: Escherichia coli

Quick links

Citations

99 review citations

Targeting cancer stem cells by inhibiting Wnt, Notch, and Hedgehog pathways.
Takebe et al. (2011)
98 more

11 mentions without citation

The canonical Notch signaling pathway: unfolding the activation mechanism.
Kopan et al. (2009)
10 more