› 2orv

X-ray diffraction
2.3Å resolution

human Thymidine Kinase 1 in complex with TP4A

Released:
DOI: 10.2210/pdb2orv/pdb
PDB entry 2orv coloured by chain, front view.
PDB entry 2orv coloured by chain, side view.
PDB entry 2orv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure.
Segura-Peña D, Lutz S, Monnerjahn C, Konrad M, Lavie A
J Mol Biol 369 129-41 (2007)
PMID: 17407781

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase, cytosolic Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04183 (Residues: 1-234; Coverage: 100%)
Gene name: TK1
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand 4TA 2 x 4TA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P3121
Unit cell:
a: 80.98Å b: 80.98Å c: 156.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.197 0.249
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Thymidine kinase 1 through the ages: a comprehensive review.
Bitter et al. (2020)
2 more

5 mentions without citation

Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure.
Segura-Peña et al. (2007)
4 more