› 2otk

Solution NMR

Structure of Alzheimer Ab peptide in complex with an engineered binding protein

Released:
DOI: 10.2210/pdb2otk/pdb
PDB entry 2otk coloured by chain, ensemble of 24 models, front view.
PDB entry 2otk coloured by chain, ensemble of 24 models, side view.
PDB entry 2otk coloured by chain, ensemble of 24 models, top view.
Source organism: engineered binding protein
Primary publication:
Stabilization of a beta-hairpin in monomeric Alzheimer's amyloid-beta peptide inhibits amyloid formation.
Hoyer W, Grönwall C, Jonsson A, Ståhl S, Härd T
Proc Natl Acad Sci U S A 105 5099-104 (2008)
PMID: 18375754

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138366 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Amyloid-beta protein 40 Chain: C
Molecule details ›
Chain: C
Length: 40 amino acids
Theoretical weight: 4.34 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P05067 (Residues: 672-711; Coverage: 5%)
Gene names: A4, AD1, APP
Sequence domains: Beta-amyloid peptide (beta-APP)
ZAb3 Affibody dimer Chains: E, F
Molecule details ›
Chains: E, F
Length: 71 amino acids
Theoretical weight: 7.82 KDa
Source organism: engineered binding protein
Expression system: Escherichia coli
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Ab initio simulated annealing
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Affibody molecules: engineered proteins for therapeutic, diagnostic and biotechnological applications.
Löfblom et al. (2010)
33 more

39 mentions without citation

Amyloid beta: structure, biology and structure-based therapeutic development.
Chen et al. (2017)
38 more