› 2ov2

X-ray diffraction
2.1Å resolution

The crystal structure of the human RAC3 in complex with the CRIB domain of human p21-activated kinase 4 (PAK4)

Released:
DOI: 10.2210/pdb2ov2/pdb
PDB entry 2ov2 coloured by chain, front view.
PDB entry 2ov2 coloured by chain, side view.
PDB entry 2ov2 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ugochukwu E, Yang X, Elkins JM, Burgess-Brown N, Bunkoczi G, Debreczeni JED, Sundstrom M, Arrowsmith CH, Weigelt J, Edwards A, von Delft F, Knapp S, Doyle DA, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
GTP + H(2)O = GDP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-132122 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 3 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 179 amino acids
Theoretical weight: 19.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60763 (Residues: 1-177; Coverage: 92%)
Gene name: RAC3
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Serine/threonine-protein kinase PAK 4 Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 35 amino acids
Theoretical weight: 4.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O96013 (Residues: 10-44; Coverage: 6%)
Gene names: KIAA1142, PAK4
Sequence domains: P21-Rho-binding domain

Ligands and Environments

4 bound ligands:
Ligand MG 8 x MG
Ligand CL 8 x CL
Ligand GCP 8 x GCP
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 50.843Å b: 73.859Å c: 133.84Å
α: 88.58° β: 87.76° γ: 70.64°
R-values:
R R work R free
0.174 0.172 0.225
Expression system: Escherichia coli

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