› 2oxz

X-ray diffraction
1.9Å resolution

Human MMP-12 in complex with two peptides PQG and IAG

Released:
DOI: 10.2210/pdb2oxz/pdb
PDB entry 2oxz coloured by chain, front view.
PDB entry 2oxz coloured by chain, side view.
PDB entry 2oxz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Snapshots of the reaction mechanism of matrix metalloproteinases.
Bertini I, Calderone V, Fragai M, Luchinat C, Maletta M, Yeo KJ
Angew Chem Int Ed Engl 45 7952-5 (2006)
PMID: 17096442

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153974 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Macrophage metalloelastase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39900 (Residues: 106-263; Coverage: 35%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)
PRO-GLN-GLY peptide Chain: Y
Molecule details ›
Chain: Y
Length: 3 amino acids
Theoretical weight: 300 Da
Source organism: Homo sapiens
Expression system: Not provided
ILE-ALA-GLY peptide Chain: X
Molecule details ›
Chain: X
Length: 3 amino acids
Theoretical weight: 259 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: C2
Unit cell:
a: 51.546Å b: 60.378Å c: 54.449Å
α: 90° β: 115.41° γ: 90°
R-values:
R R work R free
0.214 0.207 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
7 more

4 mentions without citation

Interstitial collagen catabolism.
Fields GB. (2013)
3 more