› 2oz4

X-ray diffraction
2.7Å resolution

Structural Plasticity in IgSF Domain 4 of ICAM-1 Mediates Cell Surface Dimerization

Released:
DOI: 10.2210/pdb2oz4/pdb
PDB entry 2oz4 coloured by chain, front view.
PDB entry 2oz4 coloured by chain, side view.
PDB entry 2oz4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural plasticity in Ig superfamily domain 4 of ICAM-1 mediates cell surface dimerization.
Chen X, Kim TD, Carman CV, Mi LZ, Song G, Springer TA
Proc Natl Acad Sci U S A 104 15358-63 (2007)
PMID: 17881562

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208523 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Intercellular adhesion molecule 1 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.68 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P05362 (Residues: 213-477; Coverage: 53%)
Gene name: ICAM1
Sequence domains:
Structure domains: Immunoglobulins
FAB FRAGMENT LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig heavy chain V region J558 Chain: H
Molecule details ›
Chain: H
Length: 214 amino acids
Theoretical weight: 22.77 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01757 (Residues: 1-117; Coverage: 97%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 2 x SO4
Ligand ZN 2 x ZN
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 185.396Å b: 69.342Å c: 88.173Å
α: 90° β: 112.76° γ: 90°
R-values:
R R work R free
0.209 0.204 0.253
Expression system: Cricetulus griseus

Quick links

Citations

4 review citations

ICAM-1: A master regulator of cellular responses in inflammation, injury resolution, and tumorigenesis.
Bui et al. (2020)
3 more

11 mentions without citation

Antibody structure determination using a combination of homology modeling, energy-based refinement, and loop prediction.
Zhu et al. (2014)
10 more