› 2p02

X-ray diffraction
1.21Å resolution

Crystal structure of the alpha subunit of human S-adenosylmethionine synthetase 2

Released:
DOI: 10.2210/pdb2p02/pdb
PDB entry 2p02 coloured by chain, front view.
PDB entry 2p02 coloured by chain, side view.
PDB entry 2p02 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Papagrigoriou E, Shafqat N, Rojkova A, Niessen FH, Kavanagh KL, von Delft F, Gorrec F, Ugochukwu E, Arrowsmith CH, Edwards A, Weigelt J, Sundstrom M, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-2 Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31153 (Residues: 1-395; Coverage: 100%)
Gene names: AMS2, MAT2A, MATA2
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I222
Unit cell:
a: 68.301Å b: 94.106Å c: 117.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.101 0.1 0.119
Expression system: Escherichia coli BL21(DE3)

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