› 2p15

X-ray diffraction
1.94Å resolution

Crystal structure of the ER alpha ligand binding domain with the agonist ortho-trifluoromethylphenylvinyl estradiol

Released:
DOI: 10.2210/pdb2p15/pdb
PDB entry 2p15 coloured by chain, front view.
PDB entry 2p15 coloured by chain, side view.
PDB entry 2p15 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural plasticity in the oestrogen receptor ligand-binding domain.
Nettles KW, Bruning JB, Gil G, O'Neill EE, Nowak J, Guo Y, Hughs A, Kim Y, DeSombre ER, Dilis R, Hanson RN, Joachimiak A, Greene GL
EMBO Rep 8 563-8 (2007)
PMID: 17468738

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136928 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Estrogen receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P03372 (Residues: 298-554; Coverage: 43%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 amino acids
Theoretical weight: 1.58 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 686-698; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand EZT 2 x EZT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 56.052Å b: 84.22Å c: 58.693Å
α: 90° β: 109.59° γ: 90°
R-values:
R R work R free
0.161 0.158 0.216
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Structural overview of the nuclear receptor superfamily: insights into physiology and therapeutics.
Huang et al. (2010)
6 more

47 mentions without citation

Versatility or promiscuity: the estrogen receptors, control of ligand selectivity and an update on subtype selective ligands.
Ng et al. (2014)
46 more