› 2p1l

X-ray diffraction
2.5Å resolution

Structure of the Bcl-XL:Beclin 1 complex

Released:
DOI: 10.2210/pdb2p1l/pdb
PDB entry 2p1l coloured by chain, front view.
PDB entry 2p1l coloured by chain, side view.
PDB entry 2p1l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Bcl-XL-Beclin 1 peptide complex: Beclin 1 is a novel BH3-only protein.
Oberstein A, Jeffrey PD, Shi Y
J Biol Chem 282 13123-32 (2007)
PMID: 17337444

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero hexadecamer
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-170520 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2-like protein 1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 153 amino acids
Theoretical weight: 17.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07817 (Residues: 1-209; Coverage: 66%)
Gene names: BCL2L, BCL2L1, BCLX
Sequence domains:
Beclin-1-C 37 kDa Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 31 amino acids
Theoretical weight: 3.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14457 (Residues: 107-135; Coverage: 6%)
Gene names: BECN1, GT197
Sequence domains: Beclin-1 BH3 domain, Bcl-2-interacting

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 107.94Å b: 110.59Å c: 100.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.226 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

133 review citations

The BCL-2 protein family: opposing activities that mediate cell death.
Youle et al. (2008)
132 more

26 mentions without citation

The BCL-2 family reunion.
Chipuk et al. (2010)
25 more