2pf4

X-ray diffraction
3.1Å resolution

Crystal structure of the full-length simian virus 40 small t antigen complexed with the protein phosphatase 2A Aalpha subunit

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-136552 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 589 amino acids
Theoretical weight: 65.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76MZ3 (Residues: 1-589; Coverage: 100%)
Gene name: Ppp2r1a
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Small t antigen Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 174 amino acids
Theoretical weight: 20.52 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03081 (Residues: 1-174; Coverage: 100%)
Sequence domains: T-antigen specific domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 94.711Å b: 105.501Å c: 111.937Å
α: 115.64° β: 109.55° γ: 94.11°
R-values:
R R work R free
0.247 0.244 0.304
Expression system: Escherichia coli