› 2pg7

X-ray diffraction
2.8Å resolution

Crystal Structure of Human Microsomal P450 2A6 N297Q/I300V

Released:
DOI: 10.2210/pdb2pg7/pdb
PDB entry 2pg7 coloured by chain, front view.
PDB entry 2pg7 coloured by chain, side view.
PDB entry 2pg7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into the altered substrate specificity of human cytochrome P450 2A6 mutants.
Sansen S, Hsu MH, Stout CD, Johnson EF
Arch Biochem Biophys 464 197-206 (2007)
PMID: 17540336

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2A13 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 476 amino acids
Theoretical weight: 54.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16696 (Residues: 29-494; Coverage: 94%)
Gene name: CYP2A13
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 70.889Å b: 159.389Å c: 104.101Å
α: 90° β: 91.92° γ: 90°
R-values:
R R work R free
0.275 0.235 0.289
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Substrate binding to cytochromes P450.
Isin et al. (2008)
3 more

4 mentions without citation

Structural features of cytochromes P450 and ligands that affect drug metabolism as revealed by X-ray crystallography and NMR.
Gay et al. (2010)
3 more