› 2pjy

X-ray diffraction
3Å resolution

Structural basis for cooperative assembly of the TGF-beta signaling complex

Released:
DOI: 10.2210/pdb2pjy/pdb
PDB entry 2pjy coloured by chain, front view.
PDB entry 2pjy coloured by chain, side view.
PDB entry 2pjy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cooperative assembly of TGF-beta superfamily signaling complexes is mediated by two disparate mechanisms and distinct modes of receptor binding.
Groppe J, Hinck CS, Samavarchi-Tehrani P, Zubieta C, Schuermann JP, Taylor AB, Schwarz PM, Wrana JL, Hinck AP
Mol Cell 29 157-68 (2008)
PMID: 18243111

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145356 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transforming growth factor beta-3 Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10600 (Residues: 301-412; Coverage: 29%)
Gene name: TGFB3
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines
TGF-beta receptor type-2 Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37173 (Residues: 42-149; Coverage: 20%)
Gene name: TGFBR2
Sequence domains: Transforming growth factor beta receptor 2 ectodomain
Structure domains: CD59
TGF-beta receptor type-1 Chain: C
Molecule details ›
Chain: C
Length: 79 amino acids
Theoretical weight: 8.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36897 (Residues: 33-111; Coverage: 17%)
Gene names: ALK5, SKR4, TGFBR1
Sequence domains: Activin types I and II receptor domain
Structure domains: CD59

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6522
Unit cell:
a: 66.92Å b: 66.92Å c: 254.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.242 0.297
Expression system: Escherichia coli

Quick links

Citations

51 review citations

TGFbeta in Cancer.
Massagué J. (2008)
50 more

23 mentions without citation

The DAN family: modulators of TGF-β signaling and beyond.
Nolan et al. (2014)
22 more