› 2pll

X-ray diffraction
1.9Å resolution

Crystal structure of perdeuterated human arginase I

Released:
DOI: 10.2210/pdb2pll/pdb
PDB entry 2pll coloured by chain, front view.
PDB entry 2pll coloured by chain, side view.
PDB entry 2pll coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Expression, purification, assay, and crystal structure of perdeuterated human arginase I.
Di Costanzo L, Moulin M, Haertlein M, Meilleur F, Christianson DW
Arch Biochem Biophys 465 82-9 (2007)
PMID: 17562323

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 34.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05089 (Residues: 1-322; Coverage: 100%)
Gene name: ARG1
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand ABH 2 x ABH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3
Unit cell:
a: 90.784Å b: 90.784Å c: 69.514Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.141 0.141 0.201
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Neutron protein crystallography: A complementary tool for locating hydrogens in proteins.
O'Dell et al. (2016)
3 more

2 mentions without citation

Expression, purification, assay, and crystal structure of perdeuterated human arginase I.
Di Costanzo et al. (2007)
1 more