› 2pm8

X-ray diffraction
2.8Å resolution

Crystal structure of recombinant full length human butyrylcholinesterase

Released:
DOI: 10.2210/pdb2pm8/pdb
PDB entry 2pm8 coloured by chain, front view.
PDB entry 2pm8 coloured by chain, side view.
PDB entry 2pm8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallization and X-ray structure of full-length recombinant human butyrylcholinesterase.
Ngamelue MN, Homma K, Lockridge O, Asojo OA
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 723-7 (2007)
PMID: 17768338

Function and Biology Details

Reaction catalysed: 
An acylcholine + H(2)O = choline + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cholinesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 65.21 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06276 (Residues: 29-602; Coverage: 100%)
Gene names: BCHE, CHE1
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 5 x NAG
Ligand SO4 7 x SO4
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4212
Unit cell:
a: 150.803Å b: 150.803Å c: 142.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.292
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

Molecular interactions of cholinesterases inhibitors using in silico methods: current status and future prospects.
Khan MT. (2009)
1 more

16 mentions without citation

A perspective on multi-target drug discovery and design for complex diseases.
Ramsay et al. (2018)
15 more