› 2pmv

X-ray diffraction
2.6Å resolution

Crystal Structure of Human Intrinsic Factor- Cobalamin Complex at 2.6 A Resolution

Released:
DOI: 10.2210/pdb2pmv/pdb
PDB entry 2pmv coloured by chain, front view.
PDB entry 2pmv coloured by chain, side view.
PDB entry 2pmv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human intrinsic factor: cobalamin complex at 2.6-A resolution.
Mathews FS, Gordon MM, Chen Z, Rajashankar KR, Ealick SE, Alpers DH, Sukumar N
Proc Natl Acad Sci U S A 104 17311-6 (2007)
PMID: 17954916

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cobalamin binding intrinsic factor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 399 amino acids
Theoretical weight: 43.45 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P27352 (Residues: 19-417; Coverage: 100%)
Gene names: CBLIF, GIF, IFMH
Sequence domains: Eukaryotic cobalamin-binding protein
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand B12 2 x B12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P21
Unit cell:
a: 90.1Å b: 67.3Å c: 147.7Å
α: 90° β: 96.8° γ: 90°
R-values:
R R work R free
0.213 0.213 0.249
Expression system: Komagataella pastoris

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Citations

11 review citations

Vitamin B12 transport from food to the body's cells--a sophisticated, multistep pathway.
Nielsen et al. (2012)
10 more

8 mentions without citation

Vitamin B12: unique metalorganic compounds and the most complex vitamins.
Randaccio et al. (2010)
7 more