› 2pqw

X-ray diffraction
2Å resolution

Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 17-25), trigonal form

Released:
DOI: 10.2210/pdb2pqw/pdb
PDB entry 2pqw coloured by chain, front view.
PDB entry 2pqw coloured by chain, side view.
PDB entry 2pqw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
L3MBTL1 recognition of mono- and dimethylated histones.
Min J, Allali-Hassani A, Nady N, Qi C, Ouyang H, Liu Y, MacKenzie F, Vedadi M, Arrowsmith CH
Nat Struct Mol Biol 14 1229-30 (2007)
PMID: 18026117

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163177 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lethal(3)malignant brain tumor-like protein 1 Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 37.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y468 (Residues: 268-590; Coverage: 39%)
Gene names: KIAA0681, L3MBT, L3MBTL, L3MBTL1
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.23 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 3 x ACT
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 117.013Å b: 117.013Å c: 90.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

53 review citations

Double-strand break repair: 53BP1 comes into focus.
Panier et al. (2014)
52 more

10 mentions without citation

How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
Taverna et al. (2007)
9 more