› 2pw3

X-ray diffraction
1.56Å resolution

Structure of the PDE4D-cAMP complex

Released:
DOI: 10.2210/pdb2pw3/pdb
PDB entry 2pw3 coloured by chain, front view.
PDB entry 2pw3 coloured by chain, side view.
PDB entry 2pw3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular basis for different recognition of substrates by phosphodiesterase families 4 and 10.
Wang H, Robinson H, Ke H
J Mol Biol 371 302-7 (2007)
PMID: 17582435

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3',5'-cyclic-AMP phosphodiesterase 4D Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 37.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08499 (Residues: 388-714; Coverage: 40%)
Gene names: DPDE3, PDE4D
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 57.849Å b: 81Å c: 163.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.231
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Mammalian cyclic nucleotide phosphodiesterases: molecular mechanisms and physiological functions.
Francis et al. (2011)
1 more

6 mentions without citation

Using cAMP Sensors to Study Cardiac Nanodomains.
Schleicher et al. (2018)
5 more