› 2py9

X-ray diffraction
2.56Å resolution

Protein-RNA Interaction involving KH1 domain from Human Poly(C)-Binding Protein-2

Released:
DOI: 10.2210/pdb2py9/pdb
PDB entry 2py9 coloured by chain, front view.
PDB entry 2py9 coloured by chain, side view.
PDB entry 2py9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray crystallographic and NMR studies of protein-protein and protein-nucleic acid interactions involving the KH domains from human poly(C)-binding protein-2.
Du Z, Lee JK, Fenn S, Tjhen R, Stroud RM, James TL
RNA 13 1043-51 (2007)
PMID: 17526645

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172263 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Poly(rC)-binding protein 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 73 amino acids
Theoretical weight: 8.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15366 (Residues: 11-82; Coverage: 20%)
Gene name: PCBP2
Sequence domains: KH domain
Structure domains: K Homology domain, type 1
12-mer C-rich strand of human telomeric RNA Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 nucleotides
Theoretical weight: 3.72 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 92.006Å b: 58.697Å c: 71.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Structure and function of KH domains.
Valverde et al. (2008)
9 more

9 mentions without citation

High-throughput characterization of protein-RNA interactions.
Cook et al. (2015)
8 more