› 2pze

X-ray diffraction
1.7Å resolution

Minimal human CFTR first nucleotide binding domain as a head-to-tail dimer

Released:
DOI: 10.2210/pdb2pze/pdb
PDB entry 2pze coloured by chain, front view.
PDB entry 2pze coloured by chain, side view.
PDB entry 2pze coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of a minimal human CFTR first nucleotide-binding domain as a monomer, head-to-tail homodimer, and pathogenic mutant.
Atwell S, Brouillette CG, Conners K, Emtage S, Gheyi T, Guggino WB, Hendle J, Hunt JF, Lewis HA, Lu F, Protasevich II, Rodgers LA, Romero R, Wasserman SR, Weber PC, Wetmore D, Zhang FF, Zhao X
Protein Eng Des Sel 23 375-84 (2010)
PMID: 20150177

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystic fibrosis transmembrane conductance regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 25.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13569 (Residues: 387-646; Coverage: 15%)
Gene names: ABCC7, CFTR
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 42.803Å b: 92.779Å c: 107.419Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.247
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Structural mechanisms of CFTR function and dysfunction.
Hwang et al. (2018)
12 more

24 mentions without citation

Cystic fibrosis transmembrane conductance regulator (ABCC7) structure.
Hunt et al. (2013)
23 more