› 2q12

X-ray diffraction
1.79Å resolution

Crystal Structure of BAR domain of APPL1

Released:
DOI: 10.2210/pdb2q12/pdb
PDB entry 2q12 coloured by chain, front view.
PDB entry 2q12 coloured by chain, side view.
PDB entry 2q12 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the APPL1 BAR-PH domain and characterization of its interaction with Rab5.
Zhu G, Chen J, Liu J, Brunzelle JS, Huang B, Wakeham N, Terzyan S, Li X, Rao Z, Li G, Zhang XC
EMBO J 26 3484-93 (2007)
PMID: 17581628

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DCC-interacting protein 13-alpha Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 31.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKG1 (Residues: 5-265; Coverage: 37%)
Gene names: APPL, APPL1, DIP13A, KIAA1428
Sequence domains: BAR domain of APPL family
Structure domains: Arfaptin homology (AH) domain/BAR domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 52.985Å b: 129.199Å c: 36.854Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.255
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Mechanisms shaping the membranes of cellular organelles.
Shibata et al. (2009)
22 more

66 mentions without citation

Acyl-coenzyme A binding domain containing 3 (ACBD3; PAP7; GCP60): an emerging signaling molecule.
Fan et al. (2010)
65 more