› 2q20

X-ray diffraction
1.3Å resolution

Structure of the germline Vk1 O18/O8 light chain variable domain homodimer

Released:
DOI: 10.2210/pdb2q20/pdb
PDB entry 2q20 coloured by chain, front view.
PDB entry 2q20 coloured by chain, side view.
PDB entry 2q20 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Altered dimer interface decreases stability in an amyloidogenic protein.
Baden EM, Owen BA, Peterson FC, Volkman BF, Ramirez-Alvarado M, Thompson JR
J Biol Chem 283 15853-60 (2008)
PMID: 18400753

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin kappa variable 1-33 Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 11.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01594 (Residues: 23-117; Coverage: 100%)
Gene name: IGKV1-33
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P61
Unit cell:
a: 74.265Å b: 74.265Å c: 99.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.12 0.119 0.148
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Current perspectives on cardiac amyloidosis.
Guan et al. (2012)
5 more

16 mentions without citation

Systemic amyloidoses.
Blancas-Mejía et al. (2013)
15 more