› 2q71

X-ray diffraction
1.9Å resolution

Uroporphyrinogen Decarboxylase G168R single mutant enzyme in complex with coproporphyrinogen-III

Released:
DOI: 10.2210/pdb2q71/pdb
PDB entry 2q71 coloured by chain, front view.
PDB entry 2q71 coloured by chain, side view.
PDB entry 2q71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Two novel uroporphyrinogen decarboxylase (URO-D) mutations causing hepatoerythropoietic porphyria (HEP).
Phillips JD, Whitby FG, Stadtmueller BM, Edwards CQ, Hill CP, Kushner JP
Transl Res 149 85-91 (2007)
PMID: 17240319

Function and Biology Details

Reaction catalysed: 
Uroporphyrinogen III = coproporphyrinogen + 4 CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uroporphyrinogen decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 39.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06132 (Residues: 11-366; Coverage: 97%)
Gene name: UROD
Sequence domains: Uroporphyrinogen decarboxylase (URO-D)
Structure domains: TIM Barrel

Ligands and Environments

1 bound ligand:
Ligand CP3 1 x CP3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 103.037Å b: 103.037Å c: 71.865Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.162 0.199
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Porphyria Diagnostics-Part 1: A Brief Overview of the Porphyrias.
Ramanujam et al. (2015)
1 more

2 mentions without citation

Structural aspects of enzymes involved in prokaryotic Gram-positive heme biosynthesis.
Falb et al. (2023)
1 more