› 2q7m

X-ray diffraction
4.25Å resolution

Crystal structure of human FLAP with MK-591

Released:
DOI: 10.2210/pdb2q7m/pdb
PDB entry 2q7m coloured by chain, front view.
PDB entry 2q7m coloured by chain, side view.
PDB entry 2q7m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of inhibitor-bound human 5-lipoxygenase-activating protein.
Ferguson AD, McKeever BM, Xu S, Wisniewski D, Miller DK, Yamin TT, Spencer RH, Chu L, Ujjainwalla F, Cunningham BR, Evans JF, Becker JW
Science 317 510-2 (2007)
PMID: 17600184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arachidonate 5-lipoxygenase-activating protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 161 amino acids
Theoretical weight: 18.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20292 (Residues: 1-161; Coverage: 100%)
Gene names: ALOX5AP, FLAP
Sequence domains: MAPEG family

Ligands and Environments

1 bound ligand:
Ligand 2CS 6 x 2CS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P4212
Unit cell:
a: 180.6Å b: 180.6Å c: 140.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Leukotrienes.
Peters-Golden et al. (2007)
26 more

22 mentions without citation

Enzymes of the cyclooxygenase pathways of prostanoid biosynthesis.
Smith et al. (2011)
21 more