› 2q7o

X-ray diffraction
2.9Å resolution

Structure of human purine nucleoside phosphorylase in complex with L-Immucillin-H

Released:
DOI: 10.2210/pdb2q7o/pdb
PDB entry 2q7o coloured by chain, front view.
PDB entry 2q7o coloured by chain, side view.
PDB entry 2q7o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase.
Rinaldo-Matthis A, Murkin AS, Ramagopal UA, Clinch K, Mee SP, Evans GB, Tyler PC, Furneaux RH, Almo SC, Schramm VL
J Am Chem Soc 130 842-4 (2008)
PMID: 18154341

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: E
Molecule details ›
Chain: E
Length: 289 amino acids
Theoretical weight: 32.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 1-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand IMH 1 x IMH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R32
Unit cell:
a: 143.457Å b: 143.457Å c: 166.248Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.262
Expression system: Escherichia coli

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Citations

1 review citation

From crystal to compound: structure-based antimalarial drug discovery.
Drinkwater et al. (2014)
10 other articles

2 mentions without citation

L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase.
Rinaldo-Matthis et al. (2008)
1 more