› 2qag

X-ray diffraction
4Å resolution

Crystal structure of human septin trimer 2/6/7

Released:
DOI: 10.2210/pdb2qag/pdb
PDB entry 2qag coloured by chain, front view.
PDB entry 2qag coloured by chain, side view.
PDB entry 2qag coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into filament formation by mammalian septins.
Sirajuddin M, Farkasovsky M, Hauer F, Kühlmann D, Macara IG, Weyand M, Stark H, Wittinghofer A
Nature 449 311-5 (2007)
PMID: 17637674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-171931 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Septin-2 Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15019 (Residues: 1-361; Coverage: 100%)
Gene names: DIFF6, KIAA0158, NEDD5, SEPT2, SEPTIN2
Sequence domains: Septin
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Septin-6 Chain: B
Molecule details ›
Chain: B
Length: 427 amino acids
Theoretical weight: 48.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14141 (Residues: 1-427; Coverage: 98%)
Gene names: KIAA0128, SEP2, SEPT6, SEPTIN6
Sequence domains: Septin
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Septin-7 Chain: C
Molecule details ›
Chain: C
Length: 418 amino acids
Theoretical weight: 48.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16181 (Residues: 20-437; Coverage: 96%)
Gene names: CDC10, SEPT7, SEPTIN7
Sequence domains: Septin
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P4322
Unit cell:
a: 252.515Å b: 252.515Å c: 156.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.376 0.376 0.392
Expression system: Escherichia coli

Quick links

Citations

60 review citations

Septins: the fourth component of the cytoskeleton.
Mostowy et al. (2012)
59 more

33 mentions without citation

The emerging functions of septins in metazoans.
Saarikangas et al. (2011)
32 more