› 2qel

X-ray diffraction
2.29Å resolution

Crystal structure of the highly amyloidogenic transthyretin mutant TTR G53S/E54D/L55S- heated protein

Released:
DOI: 10.2210/pdb2qel/pdb
PDB entry 2qel coloured by chain, front view.
PDB entry 2qel coloured by chain, side view.
PDB entry 2qel coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Heating of proteins as a means of improving crystallization: a successful case study on a highly amyloidogenic triple mutant of human transthyretin.
Karlsson A, Sauer-Eriksson AE
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 695-700 (2007)
PMID: 17671371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transthyretin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02766 (Residues: 21-147; Coverage: 100%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P3221
Unit cell:
a: 58.36Å b: 58.36Å c: 229.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.231 0.289
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with its Homolog AtNRP1.
Kumar et al. (2019)

3 mentions without citation

Copper mediated amyloid-β binding to Transthyretin.
Ciccone et al. (2018)
2 more