› 2qfd

X-ray diffraction
2.7Å resolution

Crystal structure of the regulatory domain of human RIG-I with bound Hg

Released:
DOI: 10.2210/pdb2qfd/pdb
PDB entry 2qfd coloured by chain, front view.
PDB entry 2qfd coloured by chain, side view.
PDB entry 2qfd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The C-terminal regulatory domain is the RNA 5'-triphosphate sensor of RIG-I.
Cui S, Eisenächer K, Kirchhofer A, Brzózka K, Lammens A, Lammens K, Fujita T, Conzelmann KK, Krug A, Hopfner KP
Mol Cell 29 169-79 (2008)
PMID: 18243112

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral innate immune response receptor RIG-I Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 145 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95786 (Residues: 802-925; Coverage: 13%)
Gene names: DDX58, RIGI
Sequence domains: C-terminal domain of RIG-I
Structure domains: RIG-I-like receptor, C-terminal regulatory domain

Ligands and Environments

1 bound ligand:
Ligand HG 10 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 97.15Å b: 76.54Å c: 137.9Å
α: 90° β: 93.07° γ: 90°
R-values:
R R work R free
0.253 0.241 0.276
Expression system: Escherichia coli

Quick links

Citations

133 review citations

Pattern recognition receptors and inflammation.
Takeuchi et al. (2010)
132 more

4 mentions without citation

Structural insights into RNA recognition and activation of RIG-I-like receptors.
Leung et al. (2012)
3 more