› 2qme

X-ray diffraction
1.75Å resolution

Crystal structure of human RAC3 in complex with CRIB domain of human p21-activated kinase 1 (PAK1)

Released:
DOI: 10.2210/pdb2qme/pdb
PDB entry 2qme coloured by chain, front view.
PDB entry 2qme coloured by chain, side view.
PDB entry 2qme coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ugochukwu E, Yang X, Elkins JM, Burgess-Brown N, Bunkoczi G, Sundstrom M, Arrowsmith CH, Weigelt J, Edwards A, von Delft F, Knapp S, Doyle D, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
GTP + H(2)O = GDP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-158089 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 3 Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 19.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60763 (Residues: 1-177; Coverage: 92%)
Gene name: RAC3
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Serine/threonine-protein kinase PAK 1 Chain: I
Molecule details ›
Chain: I
Length: 36 amino acids
Theoretical weight: 4.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13153 (Residues: 74-109; Coverage: 7%)
Gene name: PAK1
Sequence domains: P21-Rho-binding domain

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GCP 1 x GCP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 84.226Å b: 53.48Å c: 47.682Å
α: 90° β: 93.45° γ: 90°
R-values:
R R work R free
0.151 0.149 0.192
Expression systems:
  • Escherichia coli
  • Not provided

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