› 2qp3

X-ray diffraction
2.6Å resolution

Identification and Characterization of Two Amino Acids Critical for the Substrate Inhibition of SULT2A1

Released:
DOI: 10.2210/pdb2qp3/pdb
PDB entry 2qp3 coloured by chain, front view.
PDB entry 2qp3 coloured by chain, side view.
PDB entry 2qp3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification and characterization of two amino acids critical for the substrate inhibition of human dehydroepiandrosterone sulfotransferase (SULT2A1).
Lu LY, Hsieh YC, Liu MY, Lin YH, Chen CJ, Yang YS
Mol Pharmacol 73 660-8 (2008)
PMID: 18042734

Function and Biology Details

Reactions catalysed: 
3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 2A1 Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 33.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06520 (Residues: 2-285; Coverage: 100%)
Gene names: HST, STD, SULT2A1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand AOX 1 x AOX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21212
Unit cell:
a: 76.285Å b: 131.72Å c: 44.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.264
Expression system: Escherichia coli

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Citations

9 review citations

Cortisol and DHEA in development and psychopathology.
Kamin et al. (2017)
8 more