› 2qqh

X-ray diffraction
2.5Å resolution

Structure of C8a-MACPF reveals mechanism of membrane attack in complement immune defense

Released:
DOI: 10.2210/pdb2qqh/pdb
PDB entry 2qqh coloured by chain, front view.
PDB entry 2qqh coloured by chain, side view.
PDB entry 2qqh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of C8alpha-MACPF reveals mechanism of membrane attack in complement immune defense.
Hadders MA, Beringer DX, Gros P
Science 317 1552-4 (2007)
PMID: 17872444

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Complement component C8 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 37.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07357 (Residues: 133-492; Coverage: 58%)
Gene name: C8A
Sequence domains: MAC/Perforin domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I422
Unit cell:
a: 178.92Å b: 178.92Å c: 75.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.255 0.287
Expression system: Escherichia coli

Quick links

Citations

56 review citations

Complement System Part I - Molecular Mechanisms of Activation and Regulation.
Merle et al. (2015)
55 more

7 mentions without citation

Cryo-EM structure of the gasdermin A3 membrane pore.
Ruan et al. (2018)
6 more